MFI - Micro-Flow Imaging
MFI™ gives you more insight into the world of sub-visible particles—way more than just size and count. Because it's image-based, you also get quantifiable morphological parameters for your analysis. Easily differentiate and characterise different particle populations in no time.
MFI has an optic focal length that spans the entire fluidic path, so your sample data is robust and reproducible day in and day out. Add a Bot1 to automate the whole process and kick your throughput up a notch. If you're looking for broader, deeper analysis of your biotherapeutic products, MFI is the particle analysis tool for you.
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How it works
Micro-Flow Imaging combines the direct imaging capabilities of digital microscopy with the precise control of microfluidics. This results in high-resolution images with 85% sampling efficiency, more precise counts and sizing with full morphological detail for all sub-visible particles in your sample. Have complete confidence that you can accurately identify most particle type, from protein aggregates to air bubbles.
Images of the sample are captured as it passes through the flow cell's sensing zone. Every particle in every image is then analysed to create a database of particle count, size, transparency and morphology (or shape). And you'll have visual verification on the spot, as images are displayed in real-time. You can also display the results for many samples at once, which makes it easy to monitor stability and comparability.
Explore MFI – Virtual Demo
See how MFI easily detects, quantitates, and provides morphological data on subvisible particles.
MFI system specifications
DESCRIPTION |
MFI 5100 |
MFI 5200 |
| Size Range | 2 µm to 300 µm | 1 µm to 70 µm |
| Percent of Sample Analyzed | >85% – entire size range | >85% – entire size range |
| Depth of Field (DOF) | 400 µm | 100 µm |
| Flow Cell Depth | 400 µm – DOF matched | 100 µm – DOF matched |
| Analysis Rate | 200 µL/min | 150 µL/min |
| Maximum Concentration (@2.5 µm) | 175,000 particles/mL | 900,000 particles/mL |
| Automation | High Throughput Bot1 Autosampler (optional) | |
| Sample Loading Options | Pipette tip: 1 mL manual insertion, 1 mL Autosampler Syringe barrel: 2 mL, 10 mL, 20 mL manual insertion BD Hypak™ Syringe Introduction Adapter: 1 mL, 2 mL | |
| Flow Cell Coatings | Non-coated, hydrophobic, custom | |
| Precision Stirring | Variable speed control: 200–2,000 RPM Compatible with syringe barrel usage | |
| Data Output | Chart formats: histograms, scatterplots, trend charts Image formats: uncompressed TIFF (analysis), compressed JPG (storage) |
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| Particle Count Parameters | Particle count, concentration, mass, volume | |
| Morphology Parameters | Size (ECD), maximum feret diameter, aspect ratio, circularity, area, perimeter, intensity | |
| Customized Analysis Filters | Combine two or more image and count parameters in a filter, for up to 511 different custom analysis filters | |
| MFI View System Software (MVSS) See product brochure for full details | Method-based analysis protocols Time-resolved analysis and data compression for storage 21 CFR Part 11 enabling features Secure access with comprehensive audit/log files Fully compatible with MFI Image Analysis Software |
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| MFI Image Analysis Software See product brochure for full details |
Integrates with MVSS for 21 CFR Part 11 compliant analysis Review images, parametric data and trend charts Isolate sub-populations using “find-similar” function Create multi-node filters with real-time feedback Generate comprehensive reports with multiple categories Create analysis templates for repeat use |
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| Dimensions | MFI 5000 Alone: 23.97 in W X 17.01 in D X 12.00 in H Automated: 23.97 in W X 17.01 in D X 26.20 in H |
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Interested in other protein characterisation technologies? See what else Protein Simple has to offer!
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