PolyATtract mRNA Isolation System II with Magnetic Stand, 3 isolations Item ID: PMZ5200

RNA purification,RNA preps,total preps,gtc,dnase,rnase,ribonuclease,RNA expression,northerns,northern blotting,PolyA selection,rt-pcr,oligo(dT) selection,genomic DNA,guanadine thiocyanate,mrna,messenger rna,polyattract,polyatract,poly attract,poly-attract,z531,z530,z520,z521,z533,z526

The PolyATtract System isolates messenger RNA directly from total RNA.

The PolyATtract mRNA Isolation Systems use the MagneSphere technology to isolate mRNA rapidly and effectively from total RNA. The systems use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3' poly(A)+ region present in most mature eukaryotic mRNAs. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand and washed at high stringency. The mRNA is eluted from the solid phase by the simple addition of ribonuclease-free, deionized water. With total RNA as the starting material, poly(A)+ mRNA is isolated in approximately 45 minutes. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation and cDNA synthesis. Cat.# Z5200 contains sufficient reagents for 3 separate mRNA isolations, each from 1-5mg of total RNA. Cat.# Z5210 contains the same reagents as Cat.# Z5200, excluding the Magnetic Separation Stand. Cat.# Z5300 contains sufficient reagents for 15 separate mRNA isolations, each from 100-1,000ug of total RNA. Cat.# Z5310 contains the same reagents as Cat.# Z5300, excluding the Magnetic Separation Stand.