PepTag Non-Radioactive PKC Assay, 120 reactions Item ID: PMV5330

pka,cAMP dependent protein kinase,cAMP-dependent protein kinase,cAMPdependent protein kinase,kinases,protein kinase c,peptag,pep tag,pep-tag,non-radioactive,nonradioactive,non radioactive,v533,v534

The PepTag Non-Radioactive Protein Kinase Assay Systems provide a rapid, sensitive, non-radioactive method to detect either Protein Kinase C (PKC) or Protein Kinase A (PKA) activity

The PepTag Non-Radioactive Protein Kinase Assay Systems provide a rapid, sensitive and non-radioactive method to detect either Protein Kinase C (PKC) or Protein Kinase A (PKA) activity. The PepTag Assays use brightly colored, fluorescent peptide substrates that are highly specific for PKC (PepTag C1 Peptide-PLSRTLSVAAK) and PKA (PepTag A1 Peptide-LRRASLG). Phosphorylation of the peptide alters the net charge from +1 to -1. This change in the net charge allows the phosphorylated and nonphosphorylated versions of the substrate to be rapidly separated on an agarose gel at neutral pH. Using fluorescent detection, less than 2ng of purified kinase can be detected in less than 2 hours. The PepTag Non-Radioactive Protein Kinase Assay Systems can detect kinase activity in partially purified samples as well as purified preparations of enzymes, making it a good choice for the rapid screening of column fractions or the screening of kinase activators and inhibitors. In addition to the assay components, each system includes purified kinase for use as a positive control.