ProFluor Tyrosine Phosphatase Assay, 4 plate Item ID: PMV1280

PTPase,PPase,PTP-1B,PTP1b,CD45,LAR,T-cell,YOP-51,YOP51,Tcell,HTS,IMAP,molybdate,IC50,drug discovery,Leukocyte Common Antigen-Related,Yersinia outermembrane protein,profluor,pro fluor,pro-fluor,v128

ProFluor Tyrosine Phosphatase Assay measures purified tyrosine phosphatase enzyme activity in a multiwell plate format and is ideal for high-throughput applications.

The ProFluor Tyrosine Phosphatase Assay measures purified tyrosine phosphatase enzyme activity in a multiwell plate format and involves add-mix-read steps only---ideal for high-throughput applications. Tyrosine phosphatases tested with the assay include PTP-1B, CD45, LAR PTPase and YOP-51. The assay begins with a standard phosphatase reaction performed with a provided phosphorylated bisamide rhodamine 110 peptide substrate (PTPase R110 Substrate) and Control AMC Substrate that serves as a control for compounds that may inhibit the protease. Following the phosphatase reaction, a termination buffer containing a protease reagent is added, which simultaneously stops the phosphatase reaction and removes amino acids specifically from the nonphosphorylated substrate, liberating highly fluorescent rhodamine 110. Phosphorylated substrate, however, is resistant to digestion by the protease reagent and remains nonfluorescent. Thus, fluorescence intensity measured in this assay is directly correlated with phosphatase activity. The assay produces excellent Z' values (>0.7) in either 96- or 384-well plate formats and easily distinguishes known phosphatase inhibitors from other compounds.