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Polyclonal secondary antibody raised in donkey, immunoaffinity-purified using corresponding immobilized antigens and conjugated to alkaline phosphatase (AP) enzyme.
Polyclonal secondary antibody raised in donkey, immunoaffinity-purified using corresponding immobilized antigens and conjugated to alkaline phosphatase (AP) enzyme. The product (unless otherwise noted) is supplied as a 1mg/ml solution. Donkey Anti-Goat IgG, AP Conjugate is a secondary antibody developed in donkeys against goat IgG; it has been affinity-purified and conjugated to alkaline phosphatase. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. A starting working dilution of 1:2,500 is suggested for most Western blot, dot blot and ELISA applications. The optimum concentration of secondary antibody depends on the application and will need to be empirically determined.

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