r185, r188, flexi cloning system, vector cloning system
The 10X Flexi Enzyme Blend (SgfI and PmeI) is required to screen Flexi Vector clones for the presence of the protein-coding region of interest. This enzyme blend can also be used to cleave PCR products prior to cloning into the Flexi Vectors or transfer p
The 10X Flexi Enzyme Blend (SgfI and PmeI) is required to screen Flexi Vector clones for the presence of the protein-coding region of interest. This enzyme blend can also be used to cleave PCR products prior to cloning into the Flexi Vectors or transfer protein-coding regions between Flexi Vectors. For more information see the Flexi Vector Systems Technical Manual #TM254. Recognition Sites: SgfI recognition site: 5