pGEM-11Zf(+) Vector, 20microg Item ID: PMP2411

cloning,SP6 promoter,T7 promoter,pgem,pgem-11zf,pgem11zf,pgem 11zf,dual promoters,opposing promoters,in vitro transcription,transcription vector,ssDNA synthesis,p241,p242

Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.

The pGEM-11Zf(+) and pGEM-11Zf(-) Vectors can be used as standard cloning vectors, as templates for in vitro transcription and for the production of ssDNA. These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII. The pGEM-11Zf(-) and pGEM-11Zf(+) Vectors are identical except for the orientation of the f1 origin.