pGEM-9Zf(-) Vector, 20microg Item ID: PMP2391

cloning,SP6 promoter,T7 promoter,pgem,pgem-9zf,pgem9zf,pgem 9zf,dual promoters,opposing promoters,in vitro transcription,transcription vector,ssDNA synthesis,p239

Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.

The pGEM-9Zf(-) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, efficient synthesis of RNA in vitro and the production of single-stranded DNA. The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for NsiI, SpeI, HindIII, XbaI, EcoRI, SalI and SacI.