pGEM-5Zf(+) Vector, 20microg Item ID: PMP2241

cloning,SP6 promoter,T7 promoter,pgem,pgem-5zf,pgem5zf,pgem 5zf,dual promoters,opposing promoters,in vitro transcription,transcription vector,ssDNA synthesis,p224,p235

Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.

The pGEM-5Zf(+) Vector is derived from the pGEM-3Zf(+) Vector and contains the origin of replication of the filamentous phage f1. This plasmid serves as a standard cloning vector, as a templates for in vitro transcription and can be used for the production of circular ssDNA. This vector contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI. This arrangement is designed specifically for generating unidirectional deletions with the Erase-a-Base System.