pNL3.2.CMV Vector, 20microg Item ID: PMN1411

n141,luciferase,virus,sensitive,gaussia,metridia,renilla,firefly,oplophorus,reporter,nanoglo,nanoluc,cmv,hygro,hygromycin,nlucp,pest sequence,control expression vector

NanoLuc (Nluc) luciferase is a small bright luminescent reporter enzyme (19.1kDa) engineered for optimal performance. Use the pNL3.2.CMV Vector as a negative control in experiments measuring regulated changes in NanoLuc luciferase expression.

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The enzyme is about 100-fold brighter than either firefly (Photinus pyralis) or Renilla reniformis luciferase using a novel substrate, furimazine, to produce high intensity, glow-type luminescence. The luminescent reaction is ATP-independent and designed to suppress background luminescence for maximal assay sensitivity. For use as a genetic reporter, multiple forms of NanoLuc luciferase have been configured to meet differing experimental objectives. NanoLuc-PEST (NlucP) present in pNL3.2.CMV Vector closely couples protein expression to changes in transcriptional activity and increased signal-to background ratios. As a result, the pNL3.2.CMV Vector is used as a negative control for experiments configured to measure regulated changes in NanoLuc luciferase expression levels. Luminescence is linearly proportional to the amount of NanoLuc protein over a 1,000,000-fold concentration range, with a signal half-life >/=2 hours when detected with Nano-Glo Luciferase Assay Reagent. NanoLuc luciferase possesses a number of physical properties that make it an excellent reporter protein: 1) very small, monomeric enzyme (171 amino acids; 513bp); 2) high thermal stability (Tm = 60