pFC32K Nluc CMV-neo Flexi Vector, 20microg Item ID: PMN1341

luciferase, protein stability, reporter, gaussia, metridia, renilla, firefly, oplophorus, n131, n132, n133, n134, e488

The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.

The small size (19.1kDa) and extreme brightness (about 100-fold brighter than either firefly [Photinus pyralis] or Renilla reniformis) of NanoLuc luciferase (Nluc) make it an ideal protein fusion partner. NanoLuc fusion proteins can be used in a variety of applications including: reporters of protein stability, probes for bioluminescent cell imaging (BLI) or as the donor signal in bioluminescent resonance energy transfer (BRET) applications for protein:protein or protein:small-molecule interaction studies. The NanoLuc protein fusion vectors enable simple generation of N- or C-terminal fusions of NanoLuc luciferase with your protein of interest and are available. The pF Vector series generates N- or C-terminal Nluc fusion proteins using the Flexi Vector Cloning System