pNL1.3.CMV[secNluc/CMV] Vector, 20microg Item ID: PMN1101

n100,n101,n102,n103,n104,n105,n106,n107,n108,n109,n110,n111,luciferase,virus,sensitive,gaussia,metridia,renilla,firefly,oplophorus,reporter,nanoglo,nanoluc,n141,n144,n150

The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The enzyme is about 100-fold brighter than either firefly (Photinus pyralis) or Renilla reniformis luciferase using a novel substrate, furimazine, to produce high intensity, glow-type luminescence. The luminescent reaction is ATP-independent and designed to suppress background luminescence for maximal assay sensitivity. The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors are used for cloning putative regulatory elements for secreted expresson in mammalian cells. Select for stable expression in cells using hygromycin resistance. For use as a genetic reporter, multiple forms of NanoLuc luciferase have been configured to meet differing experimental objectives. Unfused Nluc offers maximal light output and sensitivity, NanoLuc-PEST (NlucP) closely couples protein expression to changes in transcriptional activity and increased signal-to background ratios, and NanoLuc luciferase fused to an N-terminal secretion signal (secNluc) is suitable when a secreted reporter is preferred. Luminescence is linearly proportional to the amount of NanoLuc protein over a 1,000,000-fold concentration range, with a signal half-life >/=2 hours when detected with Nano-Glo Luciferase Assay Reagent. NanoLuc luciferase possesses a number of physical properties that make it an excellent reporter protein: 1) very small, monomeric enzyme (171 amino acids; 513bp); 2) high thermal stability (Tm = 60