Mung Bean Nuclease, 2,000u Item ID: PMM4311

enzyme,blunt end,cloning,m431

Endonuclease specific for ssDNA.

Mung Bean Nuclease catalyzes the degradation of single-stranded DNA and RNA endonucleolytically to yield 5'-phosphoryl-terminated products. While the nuclease prefers ssDNA over dsDNA by 30,000-fold, at very high concentrations the enzyme degrades double-stranded DNA from both ends. Mung Bean Nuclease has been used for transcript mapping studies, for flushing staggered ends and for the separation of cDNA strands after synthesis with reverse transcriptase and DNA Polymerase I.