Klenow Fragment, Exonuclease Minus, 100u Item ID: PMM2181

enzyme,DNA sequencing, m218, Klenow, Klenow exo minus

Large fragment of E. coli DNA polymerase I lacking both 5' --> 3' and 3' --> 5' exonuclease activities. Suitable for manual sequencing applications.

DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus, is a DNA-dependent DNA polymerase that lacks both the 5'-->3' and the 3'-->5' exonuclease activities present in intact E. coli DNA Polymerase I. It is used for random primer labeling and in strand displacement amplification. Klenow Fragment, Exonuclease Minus, will leave a single-base 3' overhang on a significant proportion of DNA fragments during fill-in of 5'-overhangs. Therefore, this enzyme is not recommended for preparation of blunt-ended fragments for ligation.