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A complete system for phosphorylating both double- and single-stranded DNA and RNA with T4 PNK and [gamma-32P]ATP.
The DNA 5' End-Labeling System is a complete system for phosphorylating both double- and single-stranded DNA and RNA with T4 Polynucleotide Kinase and [gamma-32P]ATP. The system includes enzymes, buffers and control DNA standards to measure reaction efficiencies. Calf Intestinal Alkaline Phosphatase is included for removal of the 5'-phosphate prior to labeling with T4 Polynucleotide Kinase.