cDNA cloning,protein expression,vectors,cloning,ORF,flexivector,l106,l108,insect, insect cell extract,in vitro translation
Designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5
The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi Vectors without the need to resequence. The vectors are designed with special sequences for maximal cell-free protein expression in a specific system. The pF25A/K ICE Vectors were designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5