T Cell Activation Bioassay (NFAT), Propagation Model, 1 each Item ID: PMJ1601

biosimilar, biologic, bispecific antibody, antibody drug conjugate, potency assay, stability testing, ADCC, antibody-dependent cell-mediated cytotoxicity, cytotoxicity, target killing, effector cells, target cells, natural killer cells, NK cells, peripheral blood mononuclear cells, PBMCs, therapeutic antibody, lot release, mechanism of action, MOA, V158 (high affinity) variant, NFAT, glycosylation, fucosylation, afucosylation, Fc effector, reporter bioassay,T Cell, NFAT, IL-2

The T Cell Activation Bioassay, Propagation Model, provides a biologically relevant and specific MOA-based measure of TCR/CD3 Effector Cells without the complex workflow and variability inherent in primary cell-based assays.

The T Cell Activation Bioassay, Propagation Model, provides a biologically relevant and specific MOA-based measure of TCR/CD3 Effector Cells without the complex workflow and variability inherent in primary cell-based assays. The T Cell Activation Bioassay, Propagation Model, allows propagation and banking of the TCR/CD3 Effector Cells. Bio-Glo Luciferase Assay System (Cat.# G7940, G7941) is the required reagent for use with the T Cell Activation Bioassay, Propagation Model. The T Cell Activation Bioassays are bioluminescent cell-based assays that overcome the limitations of existing assays and can be used for the discovery and development of novel biologics such as bispecific antibodies and CAR-T cell therapies. The assays consist of a genetically engineered Jurkat T cell line that expresses a luciferase reporter (TCR/CD3 Effector Cells) driven by either an NFAT-response element (NFAT-RE) or an IL-2 promoter. When the TCR/CD3 Effector Cells (NFAT) are engaged with an appropriate TCR/CD3 ligand or anti-TCR/CD3 antibody, the TCR transduces intracellular signals resulting in NFAT-RE-mediated luminescence. Similarly, when the TCR/CD3 Effector Cells (IL-2) are co-engaged with an anti-TCR/CD3 and an anti-CD28 stimulus, receptor-mediated signaling results in IL-2 promoter-mediated luminescence.