PD-1/PD-L1 Blockade Bioassay, 1 each Item ID: PMJ1250
PD-1, PD-L1, PD-1/PD-L1, bioassay, immune checkpoint, immunotherapy, blocking antibodies, therapeutic antibodies, antibody screening, biologics, potency, stability, neutralizing antibody assay, NAb assay, DiscoverX, BPS Biosciences, reporter bioassay, luciferase reporter bioassay, functional bioassay, j125, j119, j120
The PD-1/PD-L1 Blockade Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics designed to block the PD-1/PD-L1 interaction.
PD-1 is an immune inhibitory receptor expressed on activated T cells and B cells and plays a critical role in regulating immune responses to tumor antigens and autoantigens. Engagement of PD-1 by either of its ligands, PD-L1 or PD-L2, on an adjacent cell inhibits TCR signaling and TCR-mediated proliferation, transcriptional activation and cytokine production. Therapeutic antibodies and Fc fusion proteins designed to block the PD-1/PD-L1 interaction show promising results in clinical trials for the treatment of a variety of cancers. The PD-1/PD-L1 Blockade Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics designed to block the PD-1/PD-L1 interaction. The assay consists of two genetically engineered cell lines: PD-1 Effector Cells: Jurkat T cells stably expressing human PD-1 and NFAT-induced luciferase; PD-L1 aAPC/CHO-K1 Cells: CHO-K1 cells stably expressing human PD-L1 and a cell surface protein designed to activate cognate TCRs in an antigen-independent manner. When the two cell types are co-cultured, the PD-1/PD-L1 interaction inhibits TCR signaling and NFAT-mediated luciferase activity. Addition of an antibody that blocks either PD-1 or PD-L1 releases the inhibitory signal and results in TCR signaling and NFAT-mediated luciferase activity. Product Kit Formats and Related Products: Kits are available in 1X and 5X sizes. Control Ab, Anti-PD-1 is available separately. PD-L1 Negative Cells are available separately.