RealTime-Glo MT Cell Viability Assay, 100 reactions Item ID: PMG9711

ATP cell viability assay, Cell Viability assay, Cell viability assay kit, Cell proliferation assay, Cell toxicity assay kit, Cytotoxicity Assay, Cytotoxicity assay kit, Anti-proliferative assay, In vitro tox assay, MTT assay, MTS assay, WST-1 assay, XTT assay, AlamarBlue Assay, Cyquant Assay, Live/Dead Assay, real-time viability assay, g971

The RealTime-Glo MT Cell Viability Assay is a non-lytic, homogeneous, bioluminescent method to determine in real time the number of viable cells in culture by measuring the reducing potential of cells and thus metabolism (MT).

The RealTime-Glo MT Cell Viability Assay is a non-lytic, homogeneous, bioluminescent method to determine in real time the number of viable cells in culture by measuring the reducing potential of cells and thus metabolism (MT). The assay involves adding NanoLuc luciferase and a cell-permeant pro-NanoLuc substrate to cells in culture. Viable cells reduce the proprietary pro-substrate to generate a substrate for NanoLuc luciferase. This substrate diffuses from cells into the surrounding culture medium, where it is rapidly used by the NanoLuc enzyme to produce a luminescent signal. The signal correlates with the number of viable cells, making the assay well suited for cytotoxicity studies. The reagent is stable and nontoxic to cells for up to 72 hours. No cell washing, removal of medium or further reagent addition is required to determine the number of viable cells. The non-lytic nature of this assay enables cells to be monitored over time in the same well, which reduces the amount of cells used and cell culture costs, and in downstream applications, including assay multiplexing and nucleic acid analysis.