CellTiter-Blue Cell Viability Assay, 20ml Item ID: PMG8080

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Homogeneous, fluorometric cell viability assay (resazurin). Requires a 1- to 4-hour incubation.

The CellTiter-Blue Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells rapidly lose metabolic capacity and thus do not generate a fluorescent signal. The homogeneous assay procedure involves adding the single reagent directly to cells cultured in serum-supplemented medium. After an incubation step, data are recorded using either a plate-reading fluorometer (preferred) or spectrophotometer.