PhiX174 DNA/HinfI Markers, 50microg Item ID: PMG1751

markers,ladders,lambda,phix,pgem,g171,g172,g173,g174,g175,g176

This conventional DNA digest marker is generated by ?X174 DNA digested to completion with HinfI to create 20 ethanol-precipitated DNA fragments ranging in size from 24bp to 726bp.

Conventional DNA Digest Markers are created by digesting either lambda DNA, PhiX174 replicative form DNA, or plasmids to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. The precipitated fragments are resuspended in storage buffer. The markers are not intended for quantitative analysis. Each marker is supplied with a tube of 6X Blue/Orange Loading Dye. PhiX174 DNA/HinfI Markers: Twenty ethanol-precipitated DNA fragments ranging in size from 24bp to 726bp. Recommended Loading: Load 1microl/lane.