pGEM DNA Markers, 50microg Item ID: PMG1741

markers,ladders,pgem,g174

Fifteen phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 36bp to 2,645bp. These markers are generated from separate digests of pGEM(R)-3 Vector DNA with HinfI, RsaI and AvaII and then combined.

DNA Digest Markers are created by digesting plasmids to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. The precipitated fragments are resuspended in storage buffer. The markers are not intended for quantitative analysis. Each marker is supplied with a tube of 6X Blue/Orange Loading Dye. pGEM DNA Markers: Fifteen phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 36bp to 2,645bp. These unique markers are generated from separate digests of pGEM-3 Vector DNA with HinfI, RsaI and AvaII later combined to form the markers. Recommended Loading: Load 1microl/lane.