Lambda DNA/EcoRI + HindIII Markers, 100microg Item ID: PMG1731

markers,ladders,lambda,g173,ecori,hindiii,digest

Created by digesting lambda DNA to completion with EcoRI and HindIII to generate 13 fragments of 125-21,226bp.

The Conventional DNA Digest Markers are created by digesting either lambda DNA, PhiX174 replicative form DNA, or plasmids to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. The precipitated fragments are resuspended in storage buffer. The markers are not intended for quantitative analysis. Each marker is supplied with a tube of 6X Blue/Orange Loading Dye. Lambda DNA/EcoRI + HindIII Markers: Thirteen ethanol-precipitated DNA fragments ranging in size from 125bp to 21,226bp. Recommended loading: 1microl/lane