PhiX174 DNA/HinfI Dephosphorylated Markers, 2.5microg Item ID: PME3511

DNA markers,e351

20 bands. Ready for 5' end-labeling with T4 Polynucleotide Kinase (Cat.# M4101).

PhiX174 DNA/HinfI Dephosphorylated Markers are prepared by digesting double-stranded PhiX174 DNA to completion with HinfI. The DNA fragments are then treated with calf intestinal alkaline phosphatase, phenol:chloroform-extracted, ethanol-precipitated and resuspended in TE buffer, making the markers ready for 5' end-labeling. The 20 DNA fragments range in size from 24-726bp. The markers are not intended for use in quantitative analysis. This marker is especially convenient for applications such as primer extension, requiring DNA or RNA size estimations.