Luciferase Assay Reagent, 100ml Item ID: PME1483

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An extremely sensitive reagent for rapid quantitation of firefly luciferase. Can be used for assays in single-tube luminometers or in multiwell-plate luminometers with auto-injectors.

The Luciferase Assay Reagent contains Luciferase Assay Substrate and Luciferase Assay Buffer in a convenient premixed format. The reagent is used to detect firefly luciferase activity and is a component of the Luciferase Assay System (Cat. E1500, E1501). The Luciferase Assay Reagent generates light that is nearly constant for at least 1 minute and so is compatible with measuring firefly luciferase in a single-tube luminometer or in a multiwell plate luminometer with an auto-injector. The Luciferase Assay System is a nonhomogeneous assay system; the cells containing the luciferase must be lysed before reagent addition. Glo Lysis Buffer (Cat.# E2661), Cell Culture Lysis Reagent (Cat.# E1531), Passive Lysis Buffer (Cat.# E1941) and Reporter Lysis Buffer (Cat.# E3971) can be used with the Luciferase Assay Reagent in mammalian cells. The Luciferase Assay Reagent also can be used for quantitation in plant and bacterial cells, but only Cell Culture Lysis Reagent is suitable for these applications. Reporter Lysis Buffer enables firefly luciferase, CAT and beta-galactosidase assays to be performed from the same cell extract.