pCBR-Control Vector, 20microg Item ID: PME1421

red luminescence,green luminescence,luciferase,dual color,dual reporter,HTS,high-throughput,firefly,luciferase,long half life,extended half life,drug discovery,reporter,luclite,luclight,genetic regulation,promoter definition,batch processing,continuous mode processing,click beetle,coleoptera,photinus,pyralis,luminescence,dual,renilla,two,2,transfection normalization,dual light,dual lite,dlr,reniformis,chroma-luc,chromaluc,chroma luc,chromaglo,chroma-glo,chroma glo,e142,e144,e146

Chroma-Glo Luciferase Assay System and the Chroma-Luc Vectors are used to generate red and green (dual-color) luminescence from a single sample upon addition of a single reagent.

The Chroma-Glo Luciferase Assay System and the Chroma-Luc Vectors can be used to generate red and green (dual-color) luminescence from a single sample upon addition of a single reagent. The Chroma-Luc Vectors consist of a total of six plasmids containing synthetic versions of a red or one of two green click beetle luciferase genes; CBRluc contains a red-emitting luciferase gene, while CBG68luc and CBG99luc contain green-emitting luciferase genes. Filtered measurement of the dual-color luminescence produced by the Chroma-Luc luciferases permits each reporter to be measured independently and virtually simultaneously. Besides their different luminescence colors, the three Chroma-Luc genes differ as follows: CBG99luc and CBRluc possess 99% DNA and 98% protein homology and are the ideal choice for use when working with transient expression assays; CBG68luc and CBRluc possess 68.9% DNA homology while retaining a high degree of protein homology (>98%) and thus are the preferred pair for use with stable expression assays. The Control Vectors (pCBR-Control, pCBG68-Control and pCBG99-Control Vectors) contain an SV40 promoter and enhancer for each version of the engineered click beetle genes. These control vectors are paired with a Basic Vector configuration containing a multiple cloning site (MCS) for cloning a region of interest. The Chroma-Glo Assay has a homogeneous format that generates luminescence with >30-minute signal half-lives for each of the Chroma-Luc Luciferases, thereby enabling the processing of many plates without prior sample preparation. Two reporter gene measurements can be efficiently and reproducibly determined from each well in a typical high-throughput screen.