pmirGLO Dual-Luciferase miRNA Target Expression Vector, 20microg Item ID: PME1330

miRNA,microRNA,Mir,siRNA,RNAi,Luciferase,3'-UTR,Untranslated region,Transcript stability,pmir,pmir-glo,pmir-glow,pmirglow,e133,miRNA Analysis Luciferase Vector

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3' of the firefly luciferase gene (luc2).

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3' of the firefly luciferase gene (luc2). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with luc2 used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization and selection.