pFC7A (HQ) Flexi Vector, 20microg Item ID: PMC8531

cDNA cloning,protein expression,vectors,cloning,ORF,flexivector,c851,c852,c853,c854,his tag, hq tag

These vectors are used for inducible expression of HQ--tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

The vectors below are used for inducible expression of HQ-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. The HQ tag and polyhistidine tag (His) are comparable in their affinity for Ni ions and will bind to all His-binding surfaces and resins. In certain cases the HQ-tagged proteins can be eluted from the affinity columns at lower concentrations of imidazole