pF1A T7 Flexi Vector, 20microg Item ID: PMC8441

cDNA cloning,protein expression,vectors,cloning,ORF,flexivector,c844,c845, no tag

These untagged vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Supplied as vectors used with the Flexi Cloning System.

The following vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. The Flexi Vector System is a simple, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi Vectors without the need to resequence. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector. pF1A/K T7 Flexi Vectors (Cat.# C8441, C8451) are designed for untagged protein expression. Note: Flexi Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.