Primers,cDNA,ecori,sticky ends,cloning,c1291,c129
Attach EcoRI sticky ends to blunt-ended DNA
The EcoRI Adaptors consist of two complementary oligonucleotides: a 16mer and a 12mer phosphorylated at the 5'-end. The oligonucleotides are provided annealed in equimolar concentrations in water. The EcoRI Adaptors attach EcoRI sticky ends to blunt-ended DNA.