PureYield Plasmid Midiprep Start-Up Kit, 220V Electrical (100 preps, manifold, 4 elution devices and free vacuum pump), 1 each Item ID: PMA6742

high speed plasmid isolation,rapid plasmid midiprep,qiafilter,transfection-quality plasmid,pureyield midipreps,a249,,a148,a712,a711

PureYield Plasmid Midiprep Start-Up Kit includes 100 plasmid midipreps, a vacuum manifold, 4 elution devices and a free vacuum pump. PureYield Midipreps rapidly isolate transfection-quality plasmid DNA.

The PureYield Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA. The system provides a rapid method for purification of 100-200ug of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400ug of high-copy-number plasmid from 250ml of E. coli culture. The PureYield Plasmid Midiprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method. The system is designed for use with centrifugation or vacuum (e.g., the Vac-Man Laboratory Vacuum Manifold). The PureYield Plasmid Midipreps Start-Up Kit includes 100 midipreps, a vacuum manifold, 4 elution devices and a free vacuum pump.