iPSC Reporters for Stem Cell Differentiation | ABM

As a leader in iPSC research, abm has developed adenoviral vector-based reporter systems that enable instant identification of iPSC clones with 100% transduction efficiency for all human cell types and transient expression.

abm offers both specific gene promoter-based reporters, such as those for OCT4 and Sox2, and highly sensitive, universally applicable EOS vectors.

abm provides specialised iPSC reporter systems that enable the rapid identification of pluripotent clones, making them highly effective tools for stem-cell reprogramming workflows and early-stage screening.

EOS lentiviral vectors in human ES cells. (a) EGFP expression from the indicated EOS lentiviral vectors in CA-1 human ES cell colonies cultured on MEF feeder cells (mitomycin C treated). Images were taken 3 d after infection. Scale bars, 200 mm. (b) Flow cytometry analysis of EGFP expression from the indicated vectors in human ES cells upon in vitro differentiation, as described in Figure 1c. (c) Phase contrast (left) and EGFP fluorescence (right) micrographs of EOS-EGFP expression in a representative H1 hES cell colony. Arrow: non-expressing, hES cell– derived, fibroblast-like (hdF) cells that surround the colony and represent the supportive ES cell niche. Scale bars, 200 mm
Reference: . Hotta, A et al., Nature Methods, 6(5), 370–376, 2009

Publication 1

Isolation of human IPS cells using EOS lentiviral vectors to select for pluripotency. Hotta, A et al., Nature Methods, 6(5), 370–376. click here