Immortalisation Reagents for Stable Cell Lines | ABM
Applied Biological Materials (ABM) offers specialised viral systems for generating immortalized cell lines.
Benefits:
- Extended cell lifespan
- Stable growth
- Reduced variability
Applications:
- Long-term experiments
- Disease modelling
Cell immortalisation reagents offered by abm and their application.
|
Immortalisation Strategy |
Key Reagent(s) |
Primary Cell Types |
Expression Stability |
Typical Use Case |
|
Telomere maintenance |
hTERT |
Broad |
Stable |
Long-term, physiologically relevant models |
|
Telomere + checkpoint bypass |
hTERT + CDK4 |
Epithelial cells |
Stable |
“Near-normal” immortalized cells |
|
Viral oncogene (checkpoint bypass) |
SV40 T antigen |
Broad (fibroblasts, epithelial, kidney) |
Stable |
Robust, widely used immortalization |
|
Viral oncogenes |
HPV E6/E7 |
Keratinocytes, epithelial |
Stable |
Efficient immortalization of difficult cells |
|
Viral latency |
EBV |
B lymphocytes |
Stable |
Standard B-cell immortalization |
|
Adenoviral oncogenes |
E1A–E1B |
Rodent primary cells |
Stable |
Rodent cell line generation |
|
Self-renewal programs |
HOXB8 / HOXA9 / HOXA10 |
Hematopoietic / myeloid |
Stable |
Immune & progenitor cell models |
|
Proliferative drivers |
Myc T58A, Ras V12 |
Various (specialized) |
Stable |
Rapid expansion, transformation models |
|
Tumor suppressor knockdown |
p53 / Rb siRNA |
Epithelial (adjunct) |
Transient |
Enhance hTERT immortalization |
Get Started with abm Genetic Materials
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