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The PureYield RNA Midiprep System isolates intact, pure total RNA from essentially any sample type for use in a wide range of applications.
The PureYield RNA Midiprep System isolates intact, pure total RNA from essentially any sample type for use in a wide range of applications. The use of a novel Clearing Agent enables the rapid purification of total RNA with undetectable levels of genomic DNA contamination without using DNase. A novel combination of reagents, membranes and protocol leads to yields of up to 1mg of total RNA without organic solvents, protease digestions or alcohol precipitations. One kit can be used to isolate pure total RNA from a wide variety of sample types, such as tissues, cultured cells, bacteria, yeast, plants and blood. The protocol also can be adapted for other sample types. Commonly used methods provide total RNA that is contaminated with genomic DNA. This contamination can interfere with sensitive methods, such as real-time RT-PCR and microarray analysis. The PureYield RNA Midiprep System avoids this problem by selectively removing the genomic DNA prior to total RNA purification. The eluted total RNA is free of detectable DNA and ready for use in sensitive downstream applications. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man Laboratory Vacuum Manifold) formats. The Eluator Vacuum Elution Device is used to elute nucleic acids from PureYield Midiprep or Maxiprep columns. It consists of two pieces, a blue base and a clear column assembly. The base interfaces with a Vacuum Manifold that contains Luer-Lok fittings, such as the Vac-Man Laboratory Vacuum Manifold (Cat.# A7231), and holds a 1.5ml tube to capture the eluted nucleic acids. The column assembly accepts PureYield Midiprep or Maxiprep columns. The Eluator Device eliminates the requirement for a centrifuge with a swinging bucket rotor for nucleic acid purification, simplifying and speeding purification protocols.