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Human Renal Proximal Tubule Epithelial Cells (RPTEC/TERT1) Modified to Express Drug Transporters - a New Model for Toxicity Studies

In vivo studies have shown that kidney membrane transporters play a key part in drug disposition and renal clearance. Primary renal proximal tubule epithelial cells (RPTEC) are the most physiologically relevant cell models, but lose OAT1 and OCT2 transporter expression in culture. In addition, primary RPTEC transiently expressing these transporters show large variations between production lots, making the data hard to interpret. Furthermore, cell line-based models either do not have the kidney tissue origination or the cell line itself is a cancer line.

October 20, 2017 3.00am AEST, 5.00am NZST

Presented by:
Chaozhong Zou, Ph.D. 

Senior Scientist,
ATCC Cell Systems

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Tips & Tricks to Get Publication Quality RNA ISH Data for Neuroscience Applications

RNAscope® Technology is a novel in situ hybridization (ISH) assay for detection of target RNA within intact cells. It represents a major advancement in RNA ISH approaches.
In this webinar we will discuss the benefits of both RNAscope® and BaseScope™ assays and share tips and tricks on how to perform these assays successfully to obtain publication-quality RNA in situ results.

This webinar will benefit both new and experienced RNAscope® technology users.

October 25, 2017  3am AEST | 5am NZST

Presented by:
Nidhi Vashistha, Ph.D

Technical Support Scientist
Advanced Cell Diagnostics, Inc

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Past Webinars

ACEA website logoEasy and Accurate Monitoring of Immune Cell-Mediated Cytolysis Using xCELLigence: How to Maximize Your Experimental Results

Immunotherapy is revolutionizing the field of cancer treatment, promising to provide new tools to specifically eliminate malignant cells with minimal side effects. 

Presented by:
Fabio Cerignoli, Ph.D. 
Senior Field Application Scientist
ACEA Biosciences Inc.

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ATCC logo websiteSTR Profiling for Human Cell Line Authentication

Over the past few decades, much has been written about misidentification of cell lines, primarily human cell lines. It is not unusual that studies of the ‘same’ cell lines performed by different laboratories often show different results using the same methodology. Hear Dr. Shapiro discuss the implementation of best tissue culture practices to include routine authentication of human cell lines by short tandem repeat (STR) profiling.

Presented by:
Brian Shapiro, Ph.D. Technical Writer, ATCC

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AgilentQuantifying macrophage activation using Agilent Seahorse XF technology
Metabolism is critical for normal macrophage function - upon activation there is a dramatic metabolic switch towards glycolysis.  In this webinar we will discuss new approaches for quantifying macrophage activation in real time using an Agilent XF analyzer. 

Presented by:
Brian Dranka, Ph.D.
Biology Manager, Seahorse XF Products, Agilent Technologies, Inc.

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ATCC logo websiteThe Art and Science of Growing Animal Viruses in the Laboratory: Best Practices in Virus Culture

Employing good aseptic technique and understanding the specific culture requirements of a strain are essential for optimal viral growth. In this webinar, we will discuss the basic principles for propagating animal viruses in the laboratory. Here, we will highlight best practices for making good virus stocks with an emphasis on the art and science of virus culture that should be followed to help avoid contamination and cross-contamination. Further, we will provide an in-depth look at viral culturing practices in both cell culture and embryonated chicken eggs using Influenza virus as an example.

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Measuring Calcium Flux by Flow Cytometry

Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes.
 ACEA website logo

Presented by:
Lauren Jachimowicz, Ph.D. 
Application Development Scientist, ACEA Biosciences, Inc.

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ATCC logo websiteNew isogenic cell models created by CRISPR genome editing for drug discovery
The recent development of the CRISPR/Cas9 system provides a revolutionary gene-editing technology. It is not only an advanced tool for basic research in biology but also a breakthrough technique for the development of targeted cancer therapies.

Presented by:
Fang Tian,
Ph.D., Lead Scientist, ATCC Cell Systems
Lysa-Anne Volpe, M.S., Senior Biologist, ATCC Cell Systems

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ATCC logo website Cell Health and Viability

Assessing cell viability and cell health is crucial to all aspects of cell culture. In addition to the proper maintenance of cell stocks, cell health practices increase the level of confidence in experimental results.

Presented by:
Steven Budd, M.S., MBA Product Line Business Specialist, ATCC Cell Systems

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AgilentType 1 Interferons Induce Changes in Core Metabolism that are Critical for Immune Function

Presented by:
Duojiao Wu, M.D., Ph.D. - Associate Professor in Zhongshan Hospital, Fudan University, Principle Investigator and Director of Systems Immunology. Group/Platform in Shanghai Institute of Clinical Bioinformatics

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AgilentSystems-based approach to characterizing human pluripotent stem cell metabolism

Presented by:
Hui (Sunny) Zhang - Research Professor. Zhongshan Medical School, Sun Yat-sen University in Guangzhou, China


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Agilent Metabolic reprogramming in macrophages during inflammation
Macrophages undergo profound metabolic changes when they are being activated. A major event is a switch to glycolysis which occurs in response to the bacterial product LPS. 

Presented by:
Luke O’Neill PhD FRS
Professor of Biochemistry, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin

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AgilentImmunometabolism
This webinar will explore the intricate links between immunology and metabolism.

Presented by:
Lydia Lynch - Associate Professor. Trinity College Dublin Instructor, Harvard Medical School
Christoph Hess - Professor of Medicine. University Hospital Basel
Nicholas Restifo - Senior Investigator. National Cancer Institute
Moderator: Anne Granger - Scientific Editor, Cell Metabolism

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ACEA website logoFlow Cytometry Webinar: Simplifying Assay Panel Design and WorkFLOW 
We explore the capabilities of benchtop and cost-effective flow cytometry systems and the easiest way to design flow panels.

Presented by:
Garret Guenther - Ph.D. ACEA Biosciences, Jeff Jensen - Ph.D. FluoroFinder, LLC

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ACEA website logoReal time cell analysis workshop
The workshop will be held at: 
xCELLigence Real Time Cell Analysis Workshop Lab, Theatre B, Victorian Comprehensive Cancer Centre. 

Presented by:
ACEA Biosciences & In Vitro Technologies 

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ACD Biotechne CMYKOptimize Your RNAscope® Assay Data

In this webinar we present best methods to analyze and present RNAscope® assay data.

Presented by:
Annelies Laeremans - Ph.D. Scientist, Advanced Cell Diagnostics, Inc.

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AgilentWorking Towards Defining a Metabolic Roadmap for Stem Cell Lineage Progression
Learn why metabolism is important – think beyond cellular energetics and how metabolism changes in different stem cell populations (eSCs, iPSCs and adult SCs).

Presented by:
Dr. James Ryall
Department of Physiology
The University of Melbourne

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ACD Biotechne CMYKThe Next Generation RNA In Situ Hybridization Technologies|
In Vitro Technologies are proud to invite Dr Yuling Luo, President of Advanced Cell Diagnostics to present a seminar series on Next Generation RNA In Situ Hybridization Technologies For Research and Diagnostics.

Presented By:
Dr Yuling Luo
President of Advanced Cell Diagnostics

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ATCC logo websiteATCC Microbiology Webinar – Best Practices for Stock Maintenance
Join us on Thursday, May 18 to hear Ms. Carol Horton discuss the best practices for stock maintenance with regard to passage, storage, recovery, and microbial authentication, and how ATCC manages these through the seed stock concept, adhering to the specific needs of each culture, and polyphasic strain authentication.

Presented by:
Carol Horton, M.S.
Marketing Manager, ATCC

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ACD BiotechneRNAscope® Assay Data Analysis 
Getting good conclusive results from your experiments begins with accurate analysis of your data. In this webinar, we will focus on the RNAscope® Assay and the following topics:

  • Review RNA ISH images with poor, medium and high quality targets
  • RNA ISH scoring tips RNA ISH data in comparison with IHC
  • Discuss a specific data set for HPV detection in FFPE tissue samples

Presented by:
Dr. Nancy Ciau
Board Certified Pathologist,
Advanced Cell Diagnostics, Inc.

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rnd logoNext Generation Targets for Immuno-oncology
Please join us for our webinar on Next Generation Targets for Immuno-Oncology. This webinar will discuss novel immunoregulatory pathways that we have uncovered as potential targets for oncology research and cancer immunotherapy.

Speaker:
Anthony Person, PhD
Manager in Bioassay Development
Bio-Techne

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/brands/agilent-technologies-seahorse-bioscience/Agilent Seahorse Webinar - Cell Analysis
Exploring the metabolism of CAR-T cells
In this webinar we will discuss:
- How CAR-T cells containing 4-1BB signaling domains have enforced in vitro persistence
- 4-1BB-enforced metabolic reprogramming enhances oxidative metabolism
- Mitochondrial biogenesis is selectively induced in 4-1BB CAR-T cells.

Presented by:
Roddy O'Connor, PhD
University of Pennsylvania
Center for Cellular Immunotherapies

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/brands/agilent-technologies-seahorse-bioscience/Combining Seahorse XF Analysis with stable isotope tracing to reveal novel metabolic drug targets

This webinar will discuss how merging quantitative, rate-based measurements of oxygen consumption with detailed pathway analysis using mass spectrometry have revealed the mitochondrial pyruvate carrier (MPC) as a central regulator of metabolic flexibility.

Presented by:
Ajit Divakaruni - Assistant Professor, Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA)

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ACD BiotechneGet the most out of your RNAscope® Multiplex Fluorescent assays

RNAscope® Multiplex Fluorescent assays are ideal for single-molecule detection of multiple RNA targets simultaneously within the same tissue. They are very powerful in identifying co-expression or co-localization of RNA within specific sub-populations of cells.

Presented by:
Ming Xiao He
, Ph.D, Scientist, Marie Lauigan Technical Support Scientist.

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AgilentiPSC-derived neural cells for drug discovery of mitochondrial disorders  

Mutations in mitochondrial DNA (mtDNA) cause diseases typically affecting the nervous system and for which no effective treatment exists.

Presented by:
Alessandro Prigione, M.D, Ph.D.
Independent Team Leader
Max Delbrueck Center for Molecular Medicine (MDC) in Berlin

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