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Designed for sequencing inserts cloned into the PinPoint Vectors.
The PinPoint Vector Sequencing Primer is designed for sequencing inserts cloned into the PinPoint Xa Vectors (components of Cat.# V2020). The primer hybridizes upstream of the Factor Xa site at nucleotides 325-343, approximately 40-50 base pairs upstream of the multiple cloning region and can be used to determine if an insert is cloned in-frame with the biotinylation purification tag of the PinPoint Xa Vectors. The sequence of the PinPoint Vector Sequencing Primer is 5'-d(CGTGACGCGGTGCAGGGCG)-3'. It is supplied dried.