Reagent used for the synthesis of double-stranded cDNA from mRNA and subsequent ligation into a suitable vector.
Sephacryl S-400 is a chromatography matrix used for rapid gel filtration. This matrix is useful in experiments involving the incorporation of synthetic linkers and adaptors. After linker ligation and digestion with the appropriate enzyme, unincorporated linkers and linker fragments may be rapidly removed from the DNA sample using spin columns containing Sephacryl S-400. Such columns may be used to separate small DNA fragments (</=271bp) from longer DNA molecules.