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in vitro translation,tnt,protein analysis,coupled transcription/translation,translation,lysate,l590

Consists of samples of: TNT T7 Quick for PCR DNA, TNT T7 Quick Coupled Transcription/Translation System, TNT Coupled Wheat Germ Extract System and E. coli T7 S30 Extract System for Circular DNA.

The T7 Sample System is designed to facilitate the optimization of individual gene expression by offering four unique in vitro translation systems to evaluate. The system consists of samples of: TNT T7 Quick for PCR DNA, TNT T7 Quick Coupled Transcription/Translation System, TNT Coupled Wheat Germ Extract System and E. coli T7 S30 Extract System for Circular DNA. All of the coupled systems utilize RNA generated by a T7 phage promoter. Criteria such as post-translational modifications, ionic optimization and detection methods (i.e., non-isotopic) should be considered when choosing an in vitro system. In some cases only direct experimental results will confirm which system is best for specific genes.