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insect,protein expression,baculovirus,Sf21,Sf21 cells,l110,l106,l108,cell free protein expression

The TNT T7 Insect Cell Extract Protein Expression System is a convenient, quick, single-tube, coupled transcription/translation system for cell-free protein expression.

The TNT T7 Insect Cell Extract Protein Expression System is a convenient, quick, single-tube, coupled transcription/translation system for the cell-free expression of proteins. Protein synthesis reactions are initiated by the addition of a DNA template, eliminating the need for the time-consuming process of in vitro RNA synthesis. The extract is made from the commonly used Spodoptera frugiperda Sf21 cell line. All components necessary for the transcription/translation are present in the TNT T7 ICE Master Mix. To initiate protein synthesis, the only component that must be added is the DNA template. Reactions are incubated at 28-30