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translation,IVEC,protein expression,proteonomics,proteomics,zubay,functional genomics,high-throughput,l103

The E. coli S30 Extract System for Linear Templates allows transcription/translation of linear DNA templates.

The E. coli S30 Extract System for Linear Templates allows successful transcription/translation of linear DNA templates. The investigator need only provide linear DNA containing a prokaryotic E. coli-like promoter (such as lacUV5, tac, lambdaPL (con) and lambda-P(R)). A ribosome binding site is required to direct the synthesis of proteins in vitro. In vitro-generated RNA from DNA templates lacking an E. coli promoter may also be used in this system, but protein yields will be decreased to 1-10% of that produced from linear DNA templates.