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translation,ivec,protein expression,proteonomics,proteomics,zubay,functional genomics,high-throughput,l102

E. coli S30 Extract for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors.

The E. coli S30 Extract for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors, providing a powerful tool for identifying and characterizing polypeptides. The investigator needs only to supply the cloned DNA containing the appropriate prokaryotic promoter and ribosome binding sites. The S30 Extract for Circular DNA Templates is prepared from an E. coli strain B deficient in OmpT endoproteinase and lon protease activity. This results in a greater stability of expressed proteins that would otherwise be degraded by proteases if expressed in vivo. The S30 in vitro system also allows highe expression levels of proteins that are normally expressed at low levels in vivo due to the action of host-encoded repressors.