Cell proliferation is one of the most fundamental biological processes in biology. Many of the traditional assays to measure proliferation only offer bulk population analysis but do not allow measurements of individual or mixed populations of cells.
Flow cytometry is an ideal tool to measure cell proliferation due to its quantitative measurements and ability to rapidly analyze individual cells.
New advances in the flow cytometry instrumentation and integrated data collection and analysis software not only have improved data collection and decreased hands-on time, but also enable better quality and more in-depth analysis and understanding of biologically relevant information from each sample.
Key topics to be covered:
1. Experimental conditions to stimulate PBMCs
2. Different Reagents used to measure cell proliferation
3. In-depth cell proliferation data analysis
Lauren Jachimowicz, Ph.D.
Application Development Scientist
ACEA Biosciences, Inc.
Garret Guenther, Ph.D.
Global Support Manager,
Flow Cytometry ACEA Biosciences, Inc.
Session 1: Dec. 13, 2018
9 AM, San Francisco
12 PM, New York
6 PM, Paris
4 PM Dec. 13, San Francisco
9 AM Dec. 14, Tokyo, Seoul
8 AM Dec. 14, Singapore, Beijing
Adoptive cell therapies have come of age, establishing living cells as a powerful therapeutic modality in terms of specificity, durability and adaptability. Agilent Seahorse technology is enabling the shift to real-time quantification of immune cell function, opening a new window on controlling immune cell fate and outcomes. Several translational research studies have demonstrated that altering metabolic behavior can direct immune responses and outcomes. Move beyond analyzing what your cells are, and reveal a clearer picture of what they do in order to harness their therapeutic potential.
David Ferrick, Ph.D
Cell Analysis Division - Agilent Technologies
Tuesday, December 11, 2018
08:00 PDT (Los Angeles)
11:00 EDT (New York)
16:00 BST (London)
David Ferrick, Ph.D
Cell Analysis Division
G-protein-coupled receptors (GPCRs) play a prominent role in regulating a variety of physiological function (such as sight, taste, smell), as well as fluid electrolyte balance, cardiovascular function and neurotransmission. They are the targets of more than 50% of the current therapeutic agents on the market.
Leyna Zhao, Ph.D.
Associate Director of Global Support
ACEA Biosciences, Inc
Immunotherapy is revolutionizing the field of cancer treatment, promising to provide new tools to specifically eliminate malignant cells with minimal side effects. However, the in vitro potency assays that are currently used to determine the efficacy of such reagents fail to capture the complexities and the dynamics of the immune response against cancer cells.
The xCELLigence Real-Time Cell Analysis (RTCA) platform provides a simple but sensitive approach for measuring cancer cell killing over long assay windows that aren’t accessible using other techniques such as chromium-51 release. These characteristics are of primary importance when testing reagents that are intended to act over long timescales once they are introduced inside the patient.
Fabio Cerignoli Ph.D.
STR Profiling for Human Cell Line Authentication
Over the past few decades, much has been written about misidentification of cell lines, primarily human cell lines. It is not unusual that studies of the ‘same’ cell lines performed by different laboratories often show different results using the same methodology. Hear Dr. Shapiro discuss the implementation of best tissue culture practices to include routine authentication of human cell lines by short tandem repeat (STR) profiling.
Brian Shapiro, Ph.D. Technical Writer, ATCC
Easy and Accurate Monitoring of Immune Cell-Mediated Cytolysis Using xCELLigence: How to Maximize Your Experimental Results
Immunotherapy is revolutionizing the field of cancer treatment, promising to provide new tools to specifically eliminate malignant cells with minimal side effects.
Fabio Cerignoli, Ph.D.
Senior Field Application Scientist
ACEA Biosciences Inc.
The Art and Science of Growing Animal Viruses in the Laboratory: Best Practices in Virus Culture
Employing good aseptic technique and understanding the specific culture requirements of a strain are essential for optimal viral growth. In this webinar, we will discuss the basic principles for propagating animal viruses in the laboratory. Here, we will highlight best practices for making good virus stocks with an emphasis on the art and science of virus culture that should be followed to help avoid contamination and cross-contamination. Further, we will provide an in-depth look at viral culturing practices in both cell culture and embryonated chicken eggs using Influenza virus as an example.
Quantifying macrophage activation using Agilent Seahorse XF technology
Metabolism is critical for normal macrophage function - upon activation there is a dramatic metabolic switch towards glycolysis. In this webinar we will discuss new approaches for quantifying macrophage activation in real time using an Agilent XF analyzer.
Brian Dranka, Ph.D.
Biology Manager, Seahorse XF Products, Agilent Technologies, Inc.
Measuring Calcium Flux by Flow Cytometry
Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes.
Lauren Jachimowicz, Ph.D.
Application Development Scientist, ACEA Biosciences, Inc.
New isogenic cell models created by CRISPR genome editing for drug discovery
The recent development of the CRISPR/Cas9 system provides a revolutionary gene-editing technology. It is not only an advanced tool for basic research in biology but also a breakthrough technique for the development of targeted cancer therapies.
Fang Tian, Ph.D., Lead Scientist, ATCC Cell Systems
Lysa-Anne Volpe, M.S., Senior Biologist, ATCC Cell Systems
Metabolic reprogramming in macrophages during inflammation
Macrophages undergo profound metabolic changes when they are being activated. A major event is a switch to glycolysis which occurs in response to the bacterial product LPS.
Luke O’Neill PhD FRS
Professor of Biochemistry, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin
Cell Health and Viability
Assessing cell viability and cell health is crucial to all aspects of cell culture. In addition to the proper maintenance of cell stocks, cell health practices increase the level of confidence in experimental results.
Steven Budd, M.S., MBA Product Line Business Specialist, ATCC Cell Systems
Type 1 Interferons Induce Changes in Core Metabolism that are Critical for Immune Function
Duojiao Wu, M.D., Ph.D. - Associate Professor in Zhongshan Hospital, Fudan University, Principle Investigator and Director of Systems Immunology. Group/Platform in Shanghai Institute of Clinical Bioinformatics
This webinar will explore the intricate links between immunology and metabolism.
Lydia Lynch - Associate Professor. Trinity College Dublin Instructor, Harvard Medical School
Christoph Hess - Professor of Medicine. University Hospital Basel
Nicholas Restifo - Senior Investigator. National Cancer Institute
Moderator: Anne Granger - Scientific Editor, Cell Metabolism
Working Towards Defining a Metabolic Roadmap for Stem Cell Lineage Progression
Learn why metabolism is important – think beyond cellular energetics and how metabolism changes in different stem cell populations (eSCs, iPSCs and adult SCs).
Dr. James Ryall
Department of Physiology
The University of Melbourne
The Next Generation RNA In Situ Hybridization Technologies|
In Vitro Technologies are proud to invite Dr Yuling Luo, President of Advanced Cell Diagnostics to present a seminar series on Next Generation RNA In Situ Hybridization Technologies For Research and Diagnostics.
Dr Yuling Luo
President of Advanced Cell Diagnostics
ATCC Microbiology Webinar – Best Practices for Stock Maintenance
Join us on Thursday, May 18 to hear Ms. Carol Horton discuss the best practices for stock maintenance with regard to passage, storage, recovery, and microbial authentication, and how ATCC manages these through the seed stock concept, adhering to the specific needs of each culture, and polyphasic strain authentication.
Carol Horton, M.S.
Marketing Manager, ATCC
RNAscope® Assay Data Analysis
Getting good conclusive results from your experiments begins with accurate analysis of your data. In this webinar, we will focus on the RNAscope® Assay and the following topics:
Dr. Nancy Ciau
Board Certified Pathologist,
Advanced Cell Diagnostics, Inc.
Next Generation Targets for Immuno-oncology
Please join us for our webinar on Next Generation Targets for Immuno-Oncology. This webinar will discuss novel immunoregulatory pathways that we have uncovered as potential targets for oncology research and cancer immunotherapy.
Anthony Person, PhD
Manager in Bioassay Development
Agilent Seahorse Webinar - Cell Analysis
Exploring the metabolism of CAR-T cells
In this webinar we will discuss:
- How CAR-T cells containing 4-1BB signaling domains have enforced in vitro persistence
- 4-1BB-enforced metabolic reprogramming enhances oxidative metabolism
- Mitochondrial biogenesis is selectively induced in 4-1BB CAR-T cells.
Roddy O'Connor, PhD
University of Pennsylvania
Center for Cellular Immunotherapies
Combining Seahorse XF Analysis with stable isotope tracing to reveal novel metabolic drug targets
This webinar will discuss how merging quantitative, rate-based measurements of oxygen consumption with detailed pathway analysis using mass spectrometry have revealed the mitochondrial pyruvate carrier (MPC) as a central regulator of metabolic flexibility.
Ajit Divakaruni - Assistant Professor, Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA)
Get the most out of your RNAscope® Multiplex Fluorescent assays
RNAscope® Multiplex Fluorescent assays are ideal for single-molecule detection of multiple RNA targets simultaneously within the same tissue. They are very powerful in identifying co-expression or co-localization of RNA within specific sub-populations of cells.
Ming Xiao He, Ph.D, Scientist, Marie Lauigan Technical Support Scientist.
Mutations in mitochondrial DNA (mtDNA) cause diseases typically affecting the nervous system and for which no effective treatment exists.
Alessandro Prigione, M.D, Ph.D.
Independent Team Leader
Max Delbrueck Center for Molecular Medicine (MDC) in Berlin
Mycoplasma infection can deprive host cells of nutrients and induce global changes in host gene expression, which in turn can affect data reproducibility and lead to the misinterpretation of experimental results. Best practices in cell culture coupled with routine testing is critical for the prevention and early detection of mycoplasma contamination.
Thursday, September 20, 2018
2:00 am Tuesday, in Sydney NSW
Reed Shabman, Ph.D.,
Lead Scientist, ATCC Standards Resources Center
Balsam Shawky, M.S.,
Senior Biologist, ATCC Standards Resource Center
In vivo studies have shown that kidney membrane transporters play a key part in drug disposition and renal clearance. Primary renal proximal tubule epithelial cells (RPTEC) are the most physiologically relevant cell models, but lose OAT1 and OCT2 transporter expression in culture.
Chaozhong Zou, Ph.D.
ATCC Cell Systems
RNAscope® Technology is a novel in situ hybridization (ISH) assay for detection of target RNA within intact cells. It represents a major advancement in RNA ISH approaches.
In this webinar we will discuss the benefits of both RNAscope® and BaseScope™ assays and share tips and tricks on how to perform these assays successfully to obtain publication-quality RNA in situ results.
This webinar will benefit both new and experienced RNAscope® technology users.
Nidhi Vashistha, Ph.D
Technical Support Scientist
Advanced Cell Diagnostics, Inc
Optimized cell lines are essential for modeling neurodegenerative diseases, screening novel therapeutics, and testing the neurotoxicity of environmental compounds. Neural progenitor cells (NPCs) derived from induced pluripotent stem cells are excellent in vitro models as they can be induced to differentiate down all three neural lineages.
Brian Shapiro, Ph.D.
Technical Writer, ATCC
As pluripotent stem cells become specified towards a neural stem/progenitor cell and then further differentiate into a mature neural cell type, they go through a number of molecular and metabolic changes that shift the balance between glycolysis and oxidative phosphorylation. Through the differentiation process stem cells turn off their dependence on glycolysis and move towards oxidative phosphorylation. However, whether the lineage specific gene expression drives the metabolic switch or vice versa remains unknown and is an aspect the we will further explore in this webinar.
Eva Szabo MSc, PhD
Canada Research Chair in Metabolism in Human Stem Cells and Cancer Development
Principal Investigator - Stem Cell and Cancer Research Institute (SCC-RI)
Assistant Professor - Biochemistry and Biomedical Sciences and Department of Medicine
Michael G. DeGroote School of Medicine
Creating Synthetic Protein-Protein Interaction Networks and Implications for Endogenous Network Discovery
Cells utilize networks that span both temporal and spatial organizations, encompassing many individual steps of regulation. While the regulatory regimes to build networks in synthetic biology has grown from solely transcription to also include protein or RNA modalities, circuits comprised solely of protein-protein interactions have yet to be produced.
In this webinar we will discuss:
Brian Dranka, Ph.D.
Biology Manager, Seahorse XF Products
Agilent Technologies, Inc.
Recent work has emphasized the importance of metabolic states and energy balance in maintaining stem cell function. Studies at the interface of stem cell biology and metabolism have investigated how metabolic flux is linked to changes in cell fate and dysfunction and have highlighted potential strategies to restore or manipulate stem cell metabolism for therapeutic gains in aging and disease.
In this Cell Press webinar, Alessandro Prigione (MDC), Heather Christofk (UCLA), and Michael Teitell (UCLA) will discuss their work studying metabolic regulation of pluripotency and reprogramming, and provide insights into how cellular metabolism controls self-renewal, differentiation, and survival of human pluripotent stem cells
Independent Team Leader
Associate Professor, Biological Chemistry, Molecular & Medical Pharmacology
Professor, Latta Endowed Chair and Chief of the Division of Pediatric and Neonatal Pathology
Scientific Editor, Cell Stem Cell
David Ferrick, PhD
Senior Director Marketing
Cell Analysis Division, Agilent