Results utilizing the CLARITY technique have uncovered the 3-D architecture of Alzheimer’s Disease lesions and enabled the study of the distribution of Alzheimer’s disease pathology in 3-D, even in post-mortem human tissue. Logos Biosystems have further developed the CLARITY technology to make significant improvements for tissue clearing speed, efficiency, and reproducibility. The X-CLARITY™ Tissue Clearing System from Logos Biosystems is an all-in-one, easy-to-use solution for electrophoretic tissue clearing. Its unique design accelerates the removal of lipids from tissues in a highly efficient manner, producing structurally sound and transparent whole tissues ready for multiple rounds of antibody labeling and imaging. Moreover, the X-CLARITY™ Tissue Clearing System successfully produces transparent tissues that are efficiently penetrated by and labeled with macromolecules such as antibodies or oligonucleotides. This allows for the 3D imaging of large tissues at single-cell resolution. The method has opened up a world of possibilities, from tracing neural circuitry to exploring the relationship between structure and function.
The X-CLARITY™ Tissue Clearing System can clear a whole mouse brain in just 6 hours, an astounding 8 times faster than the original technology.