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Employing non-invasive impedance microelectrodes in automated high-throughput plate formats, our xCELLigence® Real-Time Cell Analysis (RTCA) instruments maximize the physiological relevance of data extracted from in vitro cellular assays. These instruments enable label-free, real-time monitoring of cell proliferation, cell size/morphology, cell-substrate attachment quality, and cell invasion/migration. By eliminating the time- and labor-intensive steps of traditional methods, RTCA vastly improves efficiency and overall productivity. By continuously providing real-time data in short (minutes to hours) and long (days to weeks) time course experiments, RTCA provides a phenotypic view of cellular behavior at an unprecedented level of detail.

AssaysRTCA iCELLigenceRTCA DPRTCA SPRTCA MPRTCA HTRTCA CardioRTCA CardioECR
Cell Characterization / QC
Proliferation / Cytotoxicity
Adhesion
Receptor Signaling
Cell Invasion / Migration            
Cardiotoxicity          
Extracellular Recording            
Specs
Format 2×8 wells 3×16 wells 1×96 wells 6×96 wells 1×384 wells 1×96 wells 1×48 wells
Maximum Throughput 16 wells 48 wells 96 wells 576 wells 384 wells 96 wells 48 wells

rtca icelligence

RTCA iCELLigence
Wireless data transfer & large surface area wells. This instrument transmits data wirelessly from a standard CO2 cell culture incubator to the Control Unit (an iPad housed outside the incubator). The larger well size of the plates used by this model provides enough cells for complementary assays including western blotting, sequencing analyses, FACS, imaging, etc.

xCELLigence RTCA DP

xCELLigence RTCA DP
Cell invasion and migration. Capable of analyzing cell number, size/morphology, and attachment quality, with the added ability of making kinetic measurements of cell invasion and migration using an electronically integrated Boyden chamber.

xCELLigence RTCA SP

xCELLigence RTCA SP
96-well format. The SP (single plate) model differs from our other xCELLigence instruments in that it uses a single 96-well electronic microtiter plate.

xCELLigence RTCA HT

xCELLigence RTCA HT
384-well high throughput screening. Up to four 384-well instruments can be integrated and controlled by a single analyzer/control unit, giving a total of 1536 wells. Configured for high-throughput screening applications, the RTCA HT model can be used either as a standalone instrument, or integrated into a high-throughput workflow using a liquid handler for fully automated screening.

xCELLigence RTCA Cardio

xCELLigence RTCA Cardio
Cardiomyocyte contractility and viability measured in real-time. The xCELLigence® RTCA Cardio instrument expands upon ACEA’s microelectronic-based cellular assay systems to enable monitoring of cardiomyocyte beating for cardiotoxicity assessment.

xCELLigence RTCA CardioECR

xCELLigence RTCA CardioECR
Cardiomyocyte contractility, viability, and electrical activity measured in real-time. The new xCELLigence® RTCA CardioECR instrument combines high frequency measurement of cell-induced electrical impedance with multielectrode array technology to simultaneously assess cardiomyocyte contractility, viability, and electrophysiology.

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